Adenovirus-mediated stromal cell-derived-factor-1
gene transfer induces cardiac preservation after infarction via angiogenesis of CD133+ stem cells and anti-apoptosis
Junming Tang 1,
Jianing Wang 1*,
Jianye Yang 1,
Xia Kong 1
1 Yunyang Medical College, Shiyan, People's Republic of China
* To whom correspondence should be addressed. E-mail: rywjn{at}vip.163.com.
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Abstract |
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In our study, we found cardiocytes expressed CXCR4, and the number of cardiocytes apoptosis with SDF-1 treatment decreased obviously through SDF-1 induced up-regulation of phosphorylated Akt. On day 7 after myocardial infarction, marked expression of SDF-1
, and the number of CD133+ cells was the highest in the AdV-SDF-1 injection hearts. On day 28 post-treatment, blood vessel density in the AdV.SDF-1 group was higher in infracted zones. Infarct size and collagen accumulation in the infracted area decreased significantly, thickness of LV wall, vessels and cardiocytes density increased obviously in the AdV-SDF-1 group than in control or Adv-LacZ group, and hemodynamics showed the improvement of left ventricle heart function in the AdV.SDF-1 group. Therefore, SDF-1 could improve cardiac structure and function through the combined effects of angiogenesis and anti-apoptosis. Keywords: Myocardial infarction; SDF-1; Stem cell; Angiogenesis; Apoptosis
